Grupos

"EVALUATION OF A CULTURE SYSTEM FOR THE DETECTION OF MICROBIAL CONTAMINATION OF RED CELL AND PLATELET CONCENTRATES"


GABarna (genygui@terra.com.br
), Foundation Pro-Sangue Blood Center, São Paulo, Brasil; LMMimica, Department of Clinical Microbiology of Santa Casa Medical Center, São Paulo, Brasil; PEDorlhiac-Liacer, DACharmone, Foundation Pro-Sangue Blood Center, São Paulo, Brasil.

Background:
Bacterial transmission remains a significant problem in transfusion medicine and poses one of the greatest risks of morbidity and mortality for blood components transfusion recipients, particularly platelet concentrates. Despite the improved donor arm desinfection to prevent microbial contamination, this intervention is not 100% effective and, therefore, it is still necessary to screen blood donation. The Hemobac Trifásico® bacterial culture system has the potential to screen red cell and whole blood-derived platelet concentrates for the presence of bacteria with accessible cost.

Study Design and Methods:
Red cell (n=156) and whole blood-derived platelet (n=93) were evaluated. A sterile connecting device was used to attach a small transfer pack on day 2 and an American Type Culture Collection (ATCC) Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, Serratia marcescens, Escherichia coli, Propionibacterium acnes isolates were inoculate into the samples (positive controls) with variable concentrations of 7,5 and 75 CFU per mL. Either negative controls of these samples were available. After 30 minutes, 5 mL of each sample (contaminated and negative controls) were transferred into the culture bottles of a semi-automated bacteria detection system Hemobac Trifásico®, Probac of Brasil. The culture bottles were maintained for observation of a bacterial growth in the samples until 360 hours.

Results:
All microorganisms, except Propionibacterium acnes and Pseudomonas aeruginosa (7,5CFU/mL), were detected in a mean time of 24 hours wit 7,5 CFU/mL or 20 hours (75 CFU/mL). P. aeruginosa was detected in a mean time of 48 hours with 7,5 CFU/mL. P. acnes was detected in a mean time of 72 hours with 75 CFU/mL or 78 hours (7,5 CFU/mL). The 10 fold increase in inoculums was associated with a variable mean 7,7 to 16,7 percent difference in detection time (hours). None growth in the negative controls was observed after 168 and 360 hours. The positive identification of the microorganisms was verified in all contaminated samples.

Conclusion:
The Hemobac Trifásico® bacterial culture system has the potential to screen red cell and whole blood-derived platelet concentrates for the presence of aerobic and anaerobic bacteria with low cost, satisfactory sensitivity and rapid confirmation of the positive samples. Such testing can be expected to save lives.


TRANFUSION - Abstract Supplement

2005 – Vol. 45 – Page 57A


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