Grupos

"EVALUATION OF TWO DIFFERENT METHODS FOR DETECTION OF MICROBIAL CONTAMINATION IN PLATELET CONCENTRATES"

GABarna (genygui@terra.com.br), Foundation Pro-Sangue Blood Center, São Paulo, Brasil; LMMimica, Department of Clinical Microbiology of Santa Casa Medical Center, São Paulo, Brasil; PEDorlhiac-Liacer, DACharmone, Foundation Pro-Sangue Blood Center, São Paulo, Brasil."

Background:
Bacterial transmisssion remains a significant problem in transfusion medicine and poses one of the greatest risks of morbidity and mortality, particularly platelet concentrates transfusion recipients. It is still necessary to screen blood donations. The Hemobac Trifásico® bacterial culture system has the potential to screen whole blood-derived platelet concentrates for the presence of bacteria. The Radiometer Copenhagen® ABL 700 detects the presence of bacteria by measuring parameters such as: glucose, pH, pCO2, pO2 and lactate.

Study Design and Methods:
Whole blood-derived platelet (n=09) were evaluated. A sterile connecting device was used to attach a small transfer pack on day 2 after collection and an American Type Culture Collection (ATCC) S. epidermidis, S. aureus, S. marcescens, E. coli, B. cereus isolates were inoculate into the samples (positive controls) with variable concentrations of 5 and 35 CFU per 5 mL. Either negative controls of these samples were available. After 30 minutes, 5 mL of each sample (contaminated and negative controls) were transferred into the culture bottles of a semi-automated bacterial detection system Hemobac Trifásico®, Probac of Brasil. By Radiometer Copenhagen® ABL 700 measuring of concentration of glucose, pH, pCO2, pO2 and concentration of lactate was done on Day 2 and 3 after platelet collection.

Results:
All microorganisms inoculated, except Staphylococcus epidermidis, were detected in a mean time of 15,9 hours with 35 CFU/5mL or 17,9 hours (5 CFU/5mL). S. epidermidis was detected in a mean time of 25 hours with 35 CFU/5mL or in a mean time of 27 hours with 5 CFU/5mL. None growth in the negative controls was observed after 72 hours. After 48 hours, mean values observed were: pH (at 22°C) 6,96 + 0,57; pCO2 (mmHg) 45,1 + 17,8; pO2 (mmHg) 78,6 + 31,6; lactate (mmol/L) 12,9 + 6,5 and glucose (mg/dl) 291 + 81. It was observed that S. epidermidis produced low decrease in the parameters studied after 24 and 48 hours.

Conclusion:
The Hemobac Trifásico® has the potential to screen whole blood-derived platelet concentrates for the presence of bacteria with satisfactory sensitivity and rapid confirmation of the positive samples. The initial study indicates that the Radiometer Copenhagen® offers a practical and sensitive system for detecting bacteria in platelet concentrates.

TRANFUSION - Abstract Supplement
2005 – Vol. 45 – Page 57A

 

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